rapid kinetic assay – innovative
application of an approved methodology

Analytical systems based on Fluorescence intensity and polarization have been used for many decades for the investigation of many biochemical systems, including protein-protein, protein-ligand and protein-DNA interactions.

Traditional fluorescence analytical systems deploy only a small fraction of the available information – particularily the information at equilibrium or at fixed time points. The rapid kinetic assay technology instead utilizes the complete reaction time obtaining a 3 dimensional analysis of the available data. The usage of the additional information of this third dimension – time – allows a very prezise and robust way of determining concentrations.

rapid kinetic assays are based on determining the reaction kinetic of an analyte with a specific antibody or receptor. The reaction takes place in solution, the polarized fluorescent intensity is being measured.

In sharp contrast to other conventional immunoassays, the aokin method allows single testings without the need for repetitive use of standards. This in turn allows a wider and a more flexible application range. On this technological basis, a new generation of immunoassays have been developed at aokin, which are notably distinguished by their speed and simplicity. These homogeneous assays utilize a simple mix-and-read technology. Immobilization and wash steps are unnecessary.